Part:BBa_K330001

Protein secretor in Lactobacillus Plantarum

The sequence consists of signal peptide gene lp_0297, Flag-tag sequence, and two enzyme cutting sites (NdeI and HindIII). Bacteria use several pathways for protein export to the membrane, the cell wall or the medium. Many proteins follow the Sec-dependent pathway and are synthesized as precursors with an N-terminal signal peptide that directs the protein to the Sec translocation machinery. Here the signal peptide lp_0297 can help to drive the protein added after it to be secreted out of Lactobacillus Plantarum, and the signal peptide itself will be cleaved off during or shortly after the translocation.The flag-tag added is for the convenience of detection. The two enzyme cutting sites are designed for adding the sequence of proteins to be secreted. We can also choose SpeI and PstI sites to add protein sequence.

Reference: Genome-wide analysis of signal peptide functionality in Lactobacillus plantarum WCFS1(Geir Mathiesen*1, Anita Sveen1, May Bente Brurberg2, Lasse Fredriksen1, Lars Axelsson3 and Vincent GH Eijsink1).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]